Calculate The Dilution Required To Prepare A Stock Solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter Concentration 1, Concentration 2 & Volume 2 to calculate Volume 1.
Dilution Calculator
Volume (start): 0 µL
The Dilution Calculator Equation
The dilution calculator utilizes the following equation:
Concentration(start) x Volume(start) = Concentration(final) x Volume(final)
This equation is commonly abbreviated as: C1V1 = C2V2
An Example Of A Dilution Calculation Using The Dilution Calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 ?M solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 ?M, V2=20 ml and V1 is the unknown:
- Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
- Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
- Enter 20 into the Volume (final) box and select the correct unit (milliliter)
- Press calculate
- The answer of 100 microliter (0.1 ml) appears in the Volume (start) box
